While black fungus is marketed under several names, it’s technically different than the wood ear mushroom ( Auricularia auricula-judae), its botanical cousin. Keep this in mind when you’re cooking, as small amounts can go a long way. While soaking, the mushrooms expand 3–4 times in size. Before you eat it, it needs to be reconstituted in warm water for at least 1 hour. polytricha.Black fungus is usually sold in dried form. From these evidences, it is concluded that all of the 5 fusants are heterokaryon between A. By using restriction endonuclease analysis, the molecular size of mitochondrial DNA of Fu9, which had formed small fruiting-bodies, was confirmed to be 90.0kbp. On the other hand, phenoloxidase activities were not exhibited by Fu 9, 52, 58, and 63. In five fusants enzymic activity of alpha-galactosidase was observed. All of the 5 fusants were found to possess both specific bands which were present in the monokaryotic strains by analyzing electrophoretic isozyme. In paired cultures between monokaryotic strains (Mo A 11 and Mo P 16) and 5 fusants, all the fusants formed antagonistic lines against both Mo A 11 and Mo P 16. japonica Hara) sawdust-medium as compared to that on other sawdust-media (buna (Fagus crenata Blume) and mizunara (Quercus mongolica var. Five fusants exhibited superior mycelial growth on shirakamba (Betula platyphylla var. In regard to mycelial density, all of the 5 fusants were thicker compared to parental and monokaryotic strains. Concerning mycelial-growth rate, 5 fusants were similar to the parental and monokaryotic strain, Mo P 16. The optimal temperature of 5 fusants was 30 degrees centigrade in mycelial growth on a PDA medium. polytricha) and momokaryotic strains (Mo A 11 and Mo P 16). In addition, restriction endonuclease patterns of mitochondrial DNA of a fusant (Fu9) which formed small fruiting-bodies, were compared to those of parental strains (Auricularia auricula-judae and A. polytricha.įive fusants (Fu 9, 52, 58, 63 and 100) obtained by electrical protoplast fusion after treatment with two different metabolic inhibitors were investigated for mycelial-growth characteristics under various temperatures and on sawdust-medium, in paired cultures, by isozyme analysis and for biochemical test to clarify their characteristics. Five fusants (Fu 9, 52, 58, 63 and 100) obtained by electrical protoplast fusion after treatment with two different metabolic inhibitors were investigated for mycelial-growth characteristics under various temperatures and on sawdust-medium, in paired cultures, by isozyme analysis and for biochemical test to clarify their characteristics.
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